Table 3. Relevant characteristics and MALDI peptide fingerprinting data for the subset of verified proteins.
Protein entry name | SwissProt ID | MW (Da) | MW (Da) 2-DE | seq. cov. | Mascot score | match set silver staining profile |
GSTO1 | P78417 | 27833 | 30000 | 32% | 72 | CD45RA+ vs. CD45RA−, up-reg |
PRDX2 | P32119 | 21918 | 25000 | 38% | 103 | CD45RA+ vs. CD45RA−, up-reg. |
PROF1 | P07737 | 15085 | 15000 | 54% | 76 | CD45RO vs. CD45RA, down-reg. |
LIMS1 | P48059 | 38977 | 35000 | 29% | 83 | CD45RO vs. CD45RA, up-regCD45RA+ vs. CD45RA−, down-reg |
GDIR1* | P52565 | 23119 | 28000 | 47% | 103 | CD45RA+ vs. CD45RA−, up-reg. |
GDIR2 | P52566 | 22900 | 28000 | 56% | 75 | CD45RO+ vs. CD45RO−, up-reg |
SODM | P04179 | 24878 | 23000 | 47% | 125 | CD45RO vs. CD45RA, up-regCD45RO+ vs CD45RO−, down-reg |
THIO* | P10599 | 11884 | 13000 | 49% | 51 | CD45RO vs. CD45RA, down-reg. |
RA− and RO− indicate the profiling results for the respective untreated T cell subsets, RA+ and RO+ for that of H2O2-treated T cell subpopulation as outlined in the Material and methods section. Proteins are listed by their protein entry names. The * indicates, that the MALDI data for these targets were previously published in an independent proteomic profiling study of T cells using the same set of preparative gels for target identification [44].