Table 2. Plasmids predicted and validated in this study.
Montevideop S5-403-1 | Montevideop S5-403-2 | Inverness pR8-3668 | Rubislaw pA4-653 | Mississippi pA4-633 | Urbana pR8-2977 | |
Detection method | de novo | de novo & SIGI-HMM | de novo | SIGI-HMM | de novo | de novo |
Validation method | PCR and sequencing | PFGE & long range PCR | PCR and sequencing | PFGE & long range PCR | PCR and sequencing | PCR and sequencing |
Size (bp) | 53,632 | 299,644 | 121,190 | 152,728 | 122,532 | 123,020 |
GC% | 52 | 48 | 50 | 52 | 51 | 50 |
ORFs | 66 | 401 | 193 | 218 | 178 | 202 |
Replicon type | IncN2 | IncHI1 | IncI1/IncFIB | IncI1/IncFIB | IncI1/IncFIB | IncI1/IncFIB |
Plasmid stability genes | ardK, ardB, ccgC, cgAI, stbC stbA, EcoR124IIMRS | parM, parA, parB parR, parM, hipA | klcA, parB, parA, psiB, psiA, hok, relE/parE, kfrAs | parA, parB, psiB, psiA | klcA, parB, parA, psiB, psiA, hok, relE/ parE, kfrAs | klcA, parB, parA, psiB, psiA, hok, relE/parE |
Resistance genes | sul1,qacEΔ, aacC, aadA, strAB | Tn7-like (pcoE, sil, cus), sugE, nodT, ter, tetA | - | - | - | - |
Virulence genes | - | - | hlyD, eal, adhesin, pefC, ldaA, iroN | sopE, adhesin, hemolysin, serine/ threonine phosphatase | hlyD, eal, adhesin, pefC, ldaA, iroN | hlyD, eal, adhesin, fimCD |