Figure 1. Coccidioides growth cycle and study overview.

Coccidioides growth cycle in culture (A), total RNA was collected at 96 hours from hyphae and spherules, which were grown at 30°C and 39°C, respectively. Lifecycle in culture illustration adapted from Delgado et al, 2003 [11]. Samples were collected in biological triplicate and the results from C. immitis isolate RS and C. posadasii isolate C735 were compared (B).