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. 2012 Jul 20;3:285. doi: 10.3389/fphys.2012.00285

Figure 3.

Figure 3

p66-KO neurons are more resistant to DETA-NO treatment compared to WT neurons. Week-old p66-KO and WT hippocampal neurons were treated with various concentrations of DETA-NO (100, 250, and 500 μM) for 3 h. Cell counts were obtained 24 h post-treatment using Calcein AM as a viability indicator. (A) Cell viability percentages were significantly greater for the p66-KO neurons compared to the WT neurons for the tested DETA-NO concentrations. (* = p < 0.05). (B) DETA-NO concentration vs. survival percentage curves showed that the EC50 for 50% survival was approximately 220 μM for WT neurons and beyond experimental treatment concentrations for the p66-KO neurons, demonstrating that p66 elimination is associated with considerably increased resistance to DETA-NO treatment (n per treatment/genotype = 3 cultures; 4 plates/culture).