Figure 4.

p66-KO neurons show greater preservation of neuronal structure compared to WT neurons following DETA-NO treatment. Representative images of week-old p66-KO and WT neurons at 24 h post-treatment with either control media (A,B) or 500 μM DETA-NO (C,D); viable cells have been fluorescently labeled with Calcein AM. Images were taken with a laser scanning confocal microscope using a 5X objective. Note the greater percentage of viable neurons in the p66-KO culture (D) compared to the WT culture (C) (Bar in A = 50 μm).