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. 2012 Jul 20;7(7):e40986. doi: 10.1371/journal.pone.0040986

Figure 5. Crosslinking is required for elicitation of allergic ear swelling responses.

Figure 5

Mice were intradermal injected in the right ears with either 500 ng TNP-Ig-fLC (A and B) or 500 ng TNP-specific IgE (C and D). At 20 hours after sensitization, mice were intravenously injected with either 100 µg DNP-HSA (panel A and C) or 100 µg DNP-HSA combined with 750 µg DNP-Ala (50× molar excess monovalent antigen) (panel B and D). Ear swelling was measured at 10, 30 and 60 minutes after antigen injection and compared to ear thickness before antigen challenge. DNP-HSA administration caused a significant increase in ear swelling in both Ig-fLC and IgE sensitized mice (panel A and C). Challenge with DNP-HSA combined with DNP-L-Ala prevented development of ear swelling for both Ig-fLC- (panel A vs B) and IgE-mediated (panel C vs D) responses. All treatment groups consisted of 5 mice. Values are mean±SE.