Figure 3. hNPC^PSA-NCAM+ exhibited multipotency and differentiated neurons were electrophysiologically functional.

(A–D) hNPC^PSA-NCAM+ were spontaneously differentiated into neurons (A­B), oligodendrocytes (C), and astrocytes (D) in the absence of mitogens. (E) GABA-immunoreactivity was frequently observed among Tuj1-positive cells. (F–G) Voltage-dependent membrane currents: depolarizing voltage steps elicited outward K⁺ currents (F) and fast inward Na⁺ currents (G). (H–I) In current-clamp recordings, short (3 ms) or prolonged (500 ms) depolarizing current injections above supra-threshold elicited the single action potential (H), and the fast action potentials (I). Scale bars: 50 µm.