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. 2012 Jul 20;7(7):e39715. doi: 10.1371/journal.pone.0039715

Figure 4. hNPCPSA-NCAM+ were able to differentiate into specific neuronal subtypes using regionalizing cues.

Figure 4

(A) Neurons exhibiting dopaminergic phenotypes were derived from hNPCPSA-NCAM+. (B–C) The cells treated with Shh and FGF8 for 8 days, followed by treatment with BDNF, GDNF, and ascorbic acid gave rise to a larger number of TH-positive neurons than control cells (non-treated group) without a significant change in total neuronal differentiation. (D–E) TH-positive cells co-expressed with Nurr1 and Pitx3. (F) Quantitative RT-PCR analysis confirmed the enhanced expression of several transcription factors involved in dopaminergic differentiation after treatment with Shh and FGF8 to hNPCPSA-NCAM+. (G) RA/Shh-exposed hNPCPSA-NCAM+ showed induction of HoxB4 and HB9, a posterior gene and a motor neuron-specific gene, respectively. Scale bars: 20 µm, n.s., not significant, **, p<0.01.