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. 2012 Jul 20;7(7):e40935. doi: 10.1371/journal.pone.0040935

Figure 2. Crucial regions resolving HsQSOX activity.

Figure 2

(A) Crystal structure of HsQSOX1b protein. The yellow helix represents the HRR (excluding the M301-L304 region), and the cyan helix represents the ERV/ALR domain (excluding the H534-S538 region). The blue region represents M301-L304 and H534-S538. Deletion both regions acutely decrease the activity. (B) Effects of the sequence between the 534th and 556th amino acid and the sequence between the 295th and 304th on enzyme activity. The TCEP oxidase activity of the variants was relative to that of the wildtype enzyme. The TCEP oxidase activity was based on determining the rate of the H2O2 generation [22]. The truncated enzyme HsQSOX1b295–556 showed high oxidase activity to TCEP, which was slightly higher than that of the full enzyme HsQSOX1b30–604. The deletion of the sequence between the 295th and 304th amino acid greatly led to the decrease in the TCEP oxidase activity.