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. 2012 Jul 20;7(7):e40378. doi: 10.1371/journal.pone.0040378

Figure 8. mRNA levels of SPARC and SLUG in melanoma cells at various stages of tumor development.

Figure 8

(A) Analysis of SPARC and SLUG mRNA levels: relative gene expression levels of SPARC and SLUG in cultures of melanoma cells derived from RGP, VGP or metastatic melanoma tumors were evaluated by relative Q-PCR using an ABI Biosystems 7900HT Fast Real Time PCR System and the SYBR Green dye detection protocol. Data were analyzed using the 2−ddCt method and human 18S transcript level was used to normalize for each sample. Values are the mean of independent triplicates. RGP, Radial Growth Phase; VGP, Vertical Growth Phase. (B) Positive correlation between SPARC and SLUG in melanoma samples: regression analysis to determine the correlation between SPARC and SLUG in human melanoma samples. R, Spearman’s Rank Correlation Coefficient; P<0.05 (Spearman’s test).