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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Mol Immunol. 2012 Jun 4;52(3-4):165–173. doi: 10.1016/j.molimm.2012.04.013

Figure 2. The minor C allele of rs3813946 decreases proximal promoter activity.

Figure 2

(A) Transcriptional activity of luciferase constructs containing the −315/+75 proximal promoter sequence with the major T (+21T) or minor C (+21C) allele of rs3813946. Constructs were transiently transfected into CR2-negative K562 cells. Results shown represent mean transcriptional activity +SEM (n=6–9) and are expressed as normalized transcriptional activity of each construct relative to the +21T construct. * p < 0.01 when comparing normalized transcriptional activity of the +21C to the +21T construct. (B) Representation of the +21 T/C-luciferase CR2 promoter reporter constructs. Both constructs were integrated into the K562 cell line using FLP recombination. (C) K562 cells containing the stably integrated CR2 promoter constructs were isolated and luciferase activity in cell lysates was assessed. Results show mean luciferase expression ±SEM (n=5).