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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Mol Immunol. 2012 Jun 4;52(3-4):165–173. doi: 10.1016/j.molimm.2012.04.013

Figure 3. Reduced chromatin accessibility in stably transfected reporter constructs containing the minor C allele at rs3813946 correlates with transcriptional activity.

Figure 3

(A) Location of primers used to assess chromatin accessibility by CHART-PCR. (B) Pairs of “Uncut” and “Cut” samples from CHART-PCR assays were used as template in Q-PCR reactions with primer pairs targeting promoter regions of β-ACTIN (−239 to −62), PAX7 (−228 to −64), upstream CR2 (−1215 to −1052), and CR2-reporter transgene sequences (238 bp amplicon including −113 to +75 of the CR2 promoter sequence). MNase chromatin accessibility is expressed as the percentage cutting and plotted as the mean ± SEM from independent pairs of “Uncut” and “Cut” samples (n=6).