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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Mol Immunol. 2012 Jun 4;52(3-4):165–173. doi: 10.1016/j.molimm.2012.04.013

Figure 4. The T to C transition at rs3813946 alters transcription factor binding in vitro.

Figure 4

EMSA analysis of the region surrounding rs3813946. Oligonucleotides utilized correspond to +10 to +37 of the proximal promoter sequence and contained either a T (+21T) or a C (+21C) at rs3813946. Nuclear extracts from Raji B cells were pre-incubated with poly(dI-dC) in binding buffer to reduce detection of non-specific DNA-protein interactions. Increasing fold molar excess of unlabeled selfcompetitor oligonucleotide demonstrate the presence of five specific protein-DNA complexes (A–E). Unbound oligonucleotides are represented as UB.