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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Mol Immunol. 2012 Jun 4;52(3-4):165–173. doi: 10.1016/j.molimm.2012.04.013

Figure 6. Evidence that C/EBP β, PAX5 and MAZ interacts with the CR2 5` UTR in Raji cells.

Figure 6

ChIP assays demonstrate specific enrichment of CR2 5` UTR sequences in Raji cells using antibodies targeting C/EBP β, Pax5 and MAZ compared to IgG and no antibody controls. (A) Gel analysis of QPCR products from a representative ChIP assay showing PCR marker (lane 1), IgG (lane 2), C/EBP β (lane 3), PAX5 (lane 4), MAZ (lane 5), no antibody (lane 6), serially diluted inputs (lanes 7–10) and no template control (lane 11). (B) Enrichment at specific promoters was quantified using a standard curve constructed with serially diluted input samples. ChIP was performed at least three times for each antibody and the enrichment (as a percentage of input samples) at target promoters was then normalized to the background level of enrichment generated by a non-specific IgG control.