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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Bioorg Med Chem. 2012 Jun 19;20(15):4625–4634. doi: 10.1016/j.bmc.2012.06.023

Figure 2.

Figure 2

left: fluorescence emission spectra of ThT in the corresponding buffer alone (blue), monomer (red) and fibrils (green) at λex = 440 nm; right: saturation curve of ThT (3 μM) for α-synuclein fibrils (1.5 μM) in Tris buffer (30 mM, pH = 7.4) at different incubation times: 30 min (circle), 60 min (square), 90 min (triangle) at room temperature. The Kd for ThioT binding to fibrils was 948 nM and the Bmax was 5672 afu.