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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Microvasc Res. 2012 Jun 5;84(2):155–160. doi: 10.1016/j.mvr.2012.05.009

Figure 5. Time course of hydrogen peroxide concentration generated by glucose oxidase.

Figure 5

Medium was collected from wells containing human CASMC treated with glucose oxidase (5U/mL) for 2, 4, 8 and 24 h and assayed for peroxide. A large increase in hydrogen peroxide (~750 µM) was observed at 2 h and gradually declined, reaching ~250 µM by 8 h and ~60 µM by 24 h (*p<0.05, n=3, control vs. glucose oxidase).