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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: J Immunol. 2012 Jun 25;189(3):1330–1339. doi: 10.4049/jimmunol.1102757

Figure 3. The expansion of mixed human peripheral blood T cells is enhanced on soft substrates.

Figure 3

(A) Representative growth curves for total T cells, CD4+ and CD8+ T cells expanded on 1:5 and 1:50 PDMS coated with OKT3 and clone 9.3 in X-VIVO 15 culture medium. CD4+ and CD8+ T cells were enumerated by flow cytometry as described in the materials and methods. (B) The overall number of population doublings achieved for total T cells, CD4+, and CD8+ T cells stimulated by 1:5 or 1:50 PDMS coated with OKT3 and clone 9.3. Results are derived from 6 separate experiments using T cells from independent healthy donors. The 1:5 and 1:50 conditions were compared using a Paired, two-tailed Student’s t-test (*P<0.0008 for total T cells grown on a soft surface relative to hard; **P<0.0013 for CD4+ T cells grown on a soft surface relative to hard; ***P<0.0036 for CD8+ T cells grown on a soft surface relative to hard). (C) Representative data on the expression of the memory markers CD62L and CCR7 in T cell subsets prior to expansion and following expansion on PDMS substrates. The graphs to the right of the flow cytometry plots show a summary of the percent of CD4+ or CD8+ T cells lacking CD62L expression at the end of the culture period. All cultures began with a mixed population of human peripheral blood CD4+ and CD8+ T cells from 7 separate healthy donors.