Table 1.
Influence of sildenafil on the threshold for PTZ-induced seizures in mice
| Treatment (mg/kg) | CD50 of PTZ (mg/kg) | n | SEM |
|---|---|---|---|
| Control | 71.70 (61.6–83.5) | 8 | 5.57 |
| Sildenafil (5) | 69.12 (63.2–75.6) | 24 | 3.15 |
| Sildenafil (10) | 67.61 (63.9–71.5) | 24 | 1.93 |
| Sildenafil (20) | 71.96 (64.5–80.2) | 16 | 4.63 |
| Sildenafil (40) | 64.92 (56.4–74.7) | 16 | 4.64 |
Data are presented as median convulsive doses of PTZ (CD50: the dose which produces clonic seizures in 50% of animal tested) with 95% confidence limits in parentheses. Sildenafil was administered i.p. 30 min prior the s.c. administration of PTZ. CD50 values were calculated according to the log-probit method described by Litchfield and Wilcoxon (1949). One-way ANOVA was used to compare CD50 values; F(4, 83) = 0.533; p = 0.712. n total number of animals at those doses of PTZ whose convulsant effect was between 4 and 6 probits. SEM, standard error of the CD50 values