Table 4.
Effects of sildenafil and its combination with selected AEDs on motor performance, muscular strength and long-term memory in mice
| Treatment (mg/kg) | Motor impairment (%) | Retention time (s) | Muscle strength (gf) |
|---|---|---|---|
| Saline | 0 | 180 (180; 180) | 107.7 ± 5.5 |
| Sildenafil (20) | 0 | 180 (180; 180) | 108.5 ± 5.8 |
| Sildenafil (40) | 0 | 180 (180; 180) | 118.1 ± 6.9 |
| CZP (0.02) + sildenafil (20) | 0 | 180 (180; 180) | 101.9 ± 5.1 |
| CZP (0.02) + sildenafil (40) | 0 | 180 (180; 180) | 102.0 ± 6.9 |
| VPA (121.05) + sildenafil (20) | 0 | 180 (112.5; 180) | 101.0 ± 2.5 |
| VPA (101.0) + sildenafil (40) | 0 | 180 (85.5; 180) | 106.9 ± 5.7 |
| PB (13.94) + sildenafil (20) | 0 | 180 (99.75; 180) | 109.2 ± 4.3 |
| PB (13.03) +sildenafil (40) | 0 | 180 (180; 180) | 103.5 ± 5.1 |
| TGB (0.75) + sildenafil (20) | 0 | 180 (180; 180) | 102.3 ± 4.3 |
| TGB (0.75) + sildenafil (40) | 0 | 180 (180; 180) | 104.5 ± 4.9 |
| ETS (120.26) + sildenafil (20) | 0 | 180 (171; 180) | 111.8 ± 4.8 |
| ETS (72.29) + sildenafil (40) | 0 | 180 (82.5; 180) | 109.0 ± 5.0 |
Results are presented as percentage of animals showing motor coordination impairment in the chimney test in mice, as median retention times (in s; with 25th and 75th percentiles in parentheses) from the step-thorough type of passive-avoidance task, assessing long-term memory in mice, and as mean (± SEM) grip-strength in grams-force (gf) from the grip-strength test, assessing neuromuscular strength in mice. The Fisher’s exact probability test was used to analyze the results from the chimney test. Statistical analysis of data from the grip-strength test was performed with one-way ANOVA: F(12, 91) = 0.8465; p = 0.603. The results obtained in the passive-avoidance test were analyzed with nonparametric Kruskal–Wallis ANOVA: KW = 12.08; p = 0.4395. Each experimental group consisted of eight animals. All drugs were administered i.p. at times scheduled from the PTZ test and at doses corresponding to their ED50 values against clonic seizures