. Author manuscript; available in PMC: 2013 Aug 1.

Published in final edited form as: J Immunol. 2012 Jun 27;189(3):1467–1479. doi: 10.4049/jimmunol.1200079

Table I. Summary of L1O2 and ERV-K Env and Gag Immunohistochemistry Findings.

In mice only L1O2 immunohistochemistry was conducted as they lack ERV-K. Human and nonhuman primate tissue arrays were probed with all antibodies. Boxes indicate tissues staining by more than one antibody. – indicates a lack of specific staining, as defined by being greater in intensity than the isotype control and reduced upon specific but not control peptide competition.

Antigen mAb or pAb	L1O2	HERV-K Env^a,^b	HERV-K Gag^b

	RT	HERM-1811-5	p15 – 4141	p15 – 4142	CA – 4143	CA – 4144

CNS	Pituitary, Purkinje cells^c cerebrum	-	Purkinje cells (cerebellum) Neurons Ependymal cells (brain and spinal cord)			-


Pancreas	β-islets^c	-	β-islets (endocrine) Ductal epithelial cells Acini (exocrine)

Kidneys	-	Tubular epithelial cells (macula densa and proximal convoluted tubules) Medullar (collecting ducts)	Tubular epithelial cells (cortical convoluted tubules)	-	-	-
Adrenal Gland^d	Medulla (neuroendocrine cells)	-	Capsule (endocrine and stromal cells) Medulla (neuroendocrine cells)	-	-	-
♀ tissues	-	Cervical epithelium	Endometrial stroma Glandular epithelum	-	-	Cervical epithelium

♂ tissues	-	Ductal epithelium (epididymis)		-	-	-

Vasculature	-	-	Smooth muscle (media) Stromal cells (adventitia)	-	-	-
Stomach	-	-	-	Glandular epithelium	-	-
Small intestine	-	-	-	Brunner glands Enterocytes	Epithelium (crypts)	Brunner glands
Bone marrow	-	Multiple lineages (50% of precursors)	-	Brunner glands Enterocytes	Multiple lineages	Multiple lineages Megakaryocytes
Liver	-	Periportal hepatocytes	-	-	-	-
Tongue	-	Epithelium	-	-	-	-

Staining observed in non-human primate but not humans

Not tested on mouse tissues

Staining observed only in mice

Human adrenal gland samples on tissue arrays had insufficient medulla for assessment