Fig. 2.

Oct-1 binds to its site on the CRP promoter and competes with the binding of NF-κB to the overlapping κB site. A representative of three EMSA is shown. Radiolabelled WT oligo (−38 to −78) was used as the probe and nuclear extract from IL-β-treated Hep3B cells was used as the source of NF-βB. Self oligo competitor (unlabelled WT oligo) and antibodies were added to nuclear extract before the addition of the probe. DNA probe-protein complexes were visualized by using a phosphorimager. Arrows point to the complexes formed on the probe. The mobility of the free probe is not shown.