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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Mol Immunol. 2012 Jun 29;52(3-4):242–248. doi: 10.1016/j.molimm.2012.06.005

Fig. 3.

Fig. 3

Oct-1 does not bind to CRP promoter in the absence of the Oct-1 site. A representative of two EMSA is shown. Radiolabelled WT oligo (lanes 1–5) and m-Oct oligo (8 bp Oct-1 site deleted, lanes 6–10) were used as probes. Nuclear extract from untreated Hep3B cells was used as the source of constitutively active transcription factors. Self oligo competitors (unlabelled WT and m-Oct oligos) and antibodies were added to nuclear extract before the addition of the probe. DNA probe-protein complexes were visualized by using a phosphorimager. Arrows point to the complexes formed on the WT probe. The mobility of the free probe is not shown.