Fig. 6.

Oct-1 inhibits (IL-6+IL-1β)-induced CRP expression even if the Oct-1 site is deleted from the promoter. Hep3B cells were transfected with Luc-157 WT, Luc-157 m-Oct, Luc-300 WT and Luc-300 m-Oct CRP promoter constructs. One set of cells were co-transfected with the expression vector encoding Oct-1 (1 μg). After 16 h, cells were either treated with IL-6 and IL-1β or left untreated for 24 h. CRP expression was measured as Luc activity. Luc activity in the absence of Oct-1 was taken as 100%. Average ± S.E.M. of four experiments are shown. The p values for the difference between (IL-6+IL-1β) and (IL-6+IL-1β+Oct-1) groups were calculated by using paired two-tailed Students t-test (*p < 0.05, **p < 0.005). The p values for the difference between WT and corresponding m-Oct groups were calculated by using unpaired two-tailed Students t-test.