Fig. 7.

Oct-1 inhibits C/EBPβ-induced CRP expression regardless of the Oct-1 site. (A) Hep3B cells were transfected with Luc-157 WT, Luc-157 m-Oct, Luc-300 WT and Luc-300 m-Oct CRP promoter constructs. One set of cells were co-transfected with the expression vector encoding C/EBPβ (50 ng). After 40 h, CRP expression was measured as luciferase activity. Basal Luc activity was taken as 1 and C/EBPβ-induced Luc activity was plotted on the y-axis as fold over basal Luc activity. Average ± S.E.M. of four experiments are shown. Unpaired two-tailed Students t-test was used to calculate p values. (B) Hep3B cells were transfected with Luc-157 WT, Luc-157 m-Oct, Luc-300 WT and Luc-300 m-Oct CRP promoter constructs. One set of cells were co-transfected with the expression vector encoding C/EBPβ (50 ng). Another set of cells were co-transfected with the expression vectors encoding C/EBPβ (50 ng) and Oct-1 (1 μg). After 40 h, CRP expression was measured as Luc activity. Luc activity in the absence of Oct-1 was taken as 100%. Average ± S.E.M. of four experiments are shown. The p values for the difference between C/EBPβ and (C/EBPβ + Oct-1) groups were calculated by using paired two-tailed Students t-test (**p < 0.005). The p values for the difference between WT and corresponding m-Oct groups were calculated by using unpaired two-tailed Students t-test.