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. Author manuscript; available in PMC: 2013 Jul 20.
Published in final edited form as: ACS Chem Biol. 2012 Apr 20;7(7):1198–1204. doi: 10.1021/cb300024c

Figure 2.

Figure 2

Different binding between PRMT1 and PRMT1–C101A sufficient for HTS. a) Fluorescence polarization of dilutions of PRMT1 and PRMT1–C101A with maleimide–AF488 (5nM). b) Fluorescence polarization of PRMT1 and PRMT1–C101A and maleimide–AF488 as dispensed by BioRAPTR FRD. c) Fluorescence polarization Maleimide–AF488 binding to PRMT1 and PRMT1–C101A in the presence of 0.5, 1, or 2% DMSO. Data is representative of at least two independent experiments. Errors bars are SEM. *p<0.0001 d) Fluorescence polarization after sinefungin incubated for 30 minutes prior to addition of maleimide–AF488. Data is representative of two independent experiments. Errors bars are SEM. ***p<0.0001, **p<0.005, *p<0.01