Figure 5. Quantification of Uaa incorporation in luciferase shows dependence on Uaa concentration.

(A) Luciferase activities measured from strain LWA1852 (wlSi1852 [unc-54∷luciferaseTAG185 cb-unc-119 (+)]I; wlls1851[unc-54∷OmeRS_rpr-1∷ ${\text{tRNA}}_{\text{CUA}}^{\text{Tyr}}$ + myo-2∷GFP]X) grown at different OmeY concentrations. (B) Luciferase activities measured from strain LWA1031 (wlSi1852 I; wlls1565[unc-54∷DanRS_rpr-1∷ ${\text{tRNA}}_{\text{CUA}}^{\text{Leu}}$ + myo-2∷GFP]X) grown at different Ala-DanAla concentrations. Both strains were grown on solid plates at 15 °C for 1 generation in duplicate. Luminescence was normalized to total protein concentration. Error bars represent s.e.m.; n ≥ 2.