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. Author manuscript; available in PMC: 2013 Jul 20.
Published in final edited form as: ACS Chem Biol. 2012 May 11;7(7):1292–1302. doi: 10.1021/cb200542j

Figure 7. Uaa incorporation increases over multiple generations and is reversible.

Figure 7

(A) LWA1852 animals show an increase in OmeY-dependent luciferase activity over 3 generations compared to 1. (B) LWA1031 animals show an increase in Ala-DanAla-dependent luciferase activity over 3 generations compared to 1. For (A) and (B), animals were grown in duplicate on solid plates of indicated Uaa concentrations at 15 °C, and assayed after 1 or 3 generations' exposure to Uaa. (C) Comparison of multi-generation data and reversibility of Uaa incorporation. LWA1852 animals were grown in duplicate at 15 °C with 2 mM OmeY on solid plates for indicated generations. Removal of worms from the Uaa for 1 generation dropped the luciferase activity to baseline levels. All error bars represent s.e.m.; n ≥ 2.

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