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. Author manuscript; available in PMC: 2012 Jul 21.
Published in final edited form as: Cell Rep. 2012 May 24;1(6):676–688. doi: 10.1016/j.celrep.2012.04.010

Figure 2. Disruption of PERK-regulated translation in the prefrontal cortex does not alter protein synthesis.

Figure 2

(A) Left, Western blot image showing newly synthesized proteins labeled with puromycin using the SUnSET technique (see Experimental Procedures). Coronal prefrontal slices from wild-type and PERK cKO mice were treated with puromycin (5µg/mL) for 45 mins. Right, Protein synthesis levels remain unaltered between both genotypes. (cntl, no puromycin control, n=4; WT, n=4; cKO, n=4). (B) Absorbance profile (254 nm) of WT (black trace) and PERK cKO (red trace) prefrontal cortical fractions sedimented through a 20–50% linear sucrose gradient in the presence of cycloheximide.