FIG. 6.

Differentiated mESCs seeded into de-cellularized lungs in vitro show maintained expression, which increases and diversifies after implantation (A) Immunofluorescence and H&E evaluation of a normal lung, decell lung, seeded, seeded and collagen-coated, and seeded and Matrigel-coated after incubation in vitro for 24 h. Constructs were fixed and stained for Pro-SpC, TTF1, PDGFRα, and FOXJ1 after incubation. (B) Immunofluorescence and H&E evaluation of normal lung, decell lung, seeded- and implanted scaffold, collagen-coated/-seeded and collagen-implanted scaffold, and Matrigel-coated/-seeded and Matrigel-implanted scaffold. Constructs were fixed and stained for Pro-SpC, TTF1, PDGFRα, FOXJ1, and OCT4 after 14 days of subcutaneous implantation. Secondary antibody control was the same for all staining conditions. Representative images for all conditions are shown (63×oil magnification [IF] and 100×oil magnification [H&E]). (C) Graph demonstrates the positive cell count relative to the total cell count of the images found in (A). (D) Graph demonstrates the positive cell count relative to the total cell count of the images found in (B). Pro-SpC, pro-surfactant protein C; TTF1, thyroid transcription factor 1. Color images available online at www.liebertonline.com/tec