Table 1.
0.1% Triton-X100 | 2% SDC | 1 M NaCl | 30 μg/mL DNAse | |
---|---|---|---|---|
50-h protocol | 24 h | 24 h | 1 h | 1 h |
24-h protocol | 8 h | 14 h | 1 h | 1 h |
Duration of each detergent used in the 50-h and 24-h de-cellularization process is detailed above. The 24-h protocol was still effective in de-cellularizing the normal lung despite the time reduction in the first two steps.
SDC, sodium deoxycholate.