Table 1.

Reducing the 50-h De-cellularization Protocol to 24-h While Still Using the Same Reagents

	0.1% Triton-X100	2% SDC	1 M NaCl	30 μg/mL DNAse
50-h protocol	24 h	24 h	1 h	1 h
24-h protocol	8 h	14 h	1 h	1 h

Duration of each detergent used in the 50-h and 24-h de-cellularization process is detailed above. The 24-h protocol was still effective in de-cellularizing the normal lung despite the time reduction in the first two steps.

SDC, sodium deoxycholate.