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. Author manuscript; available in PMC: 2012 Dec 5.
Published in final edited form as: Eur J Pharmacol. 2011 Sep 19;671(1-3):18–25. doi: 10.1016/j.ejphar.2011.09.033

Fig. 2. Dehydroleucodine blocked the formation of lipid droplet by induction media in 3T3-L1 cells.

Fig. 2

Adipocyte differentiation was induced by treating confluent 3T3-L1 preadipocytes with induction media in the absence or presence of 8 μM DhL. Morphological changes of 3T3-L1 preadipocytes were monitored by microscope and photographed after 9 days from the onset of differentiation. (A) Vehicle only, (B) cells treated induction media in the presence DMSO, or (C) in the presence of DhL. (D) Nine days after induction of differentiation, cells were lysed for triglyceride and protein assays as described in Material and Methods. Vehicle only (line 1), cells treated with induction media alone (line 2), cells treated with induction media in the presence DMSO (line 3), and cells treated with induction media in the presence of 8 μM DhL (line 4). Bars=10μm. Data represent the mean ± S.E.M. of three independent experiments. *P < 0.05 by Student's t-test compared to DMSO-treated cells and only induction media-treated cells.