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. Author manuscript; available in PMC: 2012 Dec 5.
Published in final edited form as: Eur J Pharmacol. 2011 Sep 19;671(1-3):18–25. doi: 10.1016/j.ejphar.2011.09.033

Fig. 3. Dehydroleucodine attenuated the expression of PPARγ during 3T3-L1 preadipocyte differentiation.

Fig. 3

3T3-L1 preadipocytes were induced to differentiate by induction media into adipocytes in the absence (insert: –DhL, control) or in the presence (insert: +DhL) of 8 μM DhL. Total protein extracts were prepared at day 9 from each sample. The proteins were subset to 12% SDS-PAGE electrophoresis, blotted to a nitrocellulose membrane, and probed with anti-bodies specific to (A) PPARγ, C-EBPα and tubulin, (B) P-Erk1/2, T-Erk1/2, (C) P-Akt1, T-Akt1, and (D) P-AMPKα and T-AMPKα respectively. Relative levels of proteins were determined by densitometry as describe in Material and Methods. Data represent the mean ± S.E.M. of three independent experiments. *P < 0.05 by Student's t-test compared to DMSO induction media-treated cells (control).