Figure 2.

Ago2-independent activity is sequence specific. (A) two and four base mismatches or scrambled control siRNAs were designed based upon the sequence of siRNA 383281 (upper strand). Mismatches are depicted in lower case on the bottom strand. (B) Levels of Il4R-α (solid bars), cyclophilin (open bars), and GAPDH (striped bars) mRNA were measured by qRT/PCR 48 h post-transfection with 100 nM 383281 and control siRNAs. (C) Ago2^−/− fibroblasts were transfected with siRNA 383281 at concentrations ranging from 300 pM to 100 nM and with mismatch and scrambled controls at 100 nM. Western blot analysis was performed 24 h post-transfection as detailed in ‘Materials and Methods’ section. A total of 50 µg protein was loaded in each lane.