Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Mar 15;40(13):6223–6234. doi: 10.1093/nar/gks239

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 3. — Il4R-α siRNA activity is also independent of Ago 1, 3, and 4. Wild-type and Ago2^−/− MEFs were treated with ASOs targeting Ago1, Ago3, or Ago4. The following day Ago-reduced cells were seeded in 96-well plates then transfected with PTEN or Il4R-α siRNA at concentrations ranging from 300 pM to 300 nM. Messenger RNA reduction was measured after 24 h by qRT/PCR. IC50 curves are plotted versus no siRNA control for each treatment condition. (A) Activity of PTEN siRNA 341391 in wild-type (WT) and Ago2^−/− (KO) cells. (B) Activity of Il4R-α siRNA 383281 in WT and KO MEF cells. (C) Activity of PTEN siRNA 341391 in KO cells (circle), and KO cells reduced in Ago 1 (inverted triangle), Ago 3 (filled circle), or Ago 4 (filled triangle). (D) Activity of Il4R-α siRNA 383281 in KO cells (circle), and KO cells reduced in Ago 1 (inverted triangle), Ago 3 (filled circle), or Ago 4 (filled triangle).