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. 2012 Mar 15;40(13):6223–6234. doi: 10.1093/nar/gks239

Figure 5.

Figure 5.

Il4R-α siRNA activity does not compete for free RISC with other siRNAs. (A) WT MEF cells were transfected 10 nM Eg5 siRNA alone or in the presence of 10, 30, or 100 nM PTEN siRNA for 4 h. Reduction of Eg5 mRNA was assessed the following by qRT/PCR and plotted as percent control in untreated cells. (B) WT MEF cells were transfected 10 nM Il4R-α siRNA 383281 alone or in the presence of 10, 30, or 100 nM PTEN siRNA for 4 h. Reduction of Il4R-α mRNA was assessed the following by qRT/PCR and plotted as percent control in untreated. (C) Ago2−/− MEF cells transfected with 10 nM Il4R-α siRNA 383281 alone or in the presence of 10, 30, or 100 nM PTEN siRNA for 4 h.