Figure 8.

Treatment Il4R-α siRNA 383281 results in a small increase in polyadenylation at a downstream polyA site. (A) PolyA site and RACE primer localization on the Il4R-α transcript. Nucleotide position of 3′ RACE forward primer (FP), and polyA sites is indicated. Expected length of RACE products is given below the transcript. (B) Ago2^−/− MEF cells were treated with Il4R-α siRNA 383281 or the corresponding full MOE ASO at a concentration of 100 nM. 3′ RACE was performed using total RNA purified from the cells the following day. Left Panel, ethidium bromide stained gel. Center panel, Southern blot of RACE gel probed with Prb1. Right panel, Southern blot of RACE gel probed with Prb2.