Table 1.
Sequences of siRNAs and antisense oligonucleotides used in study
| ISIS No. | Target | Sequence | Chemistry |
|---|---|---|---|
| 383266 | Il4R-α | ACCTCAGCAACAACAGCAC | siRNA |
| 383268 | Il4R-α | GTGAAGGATCTGCTCCCAG | siRNA |
| 383270 | Il4R-α | CACAGACCTCAGCAACAAC | siRNA |
| 383273 | Il4R-α | TTCTGGGAAACCTGCCAGC | siRNA |
| 383275 | Il4R-α | GTTCTCAGGTGACATGCTC | siRNA |
| 383281 | Il4R-α | TTTATTGACATAAAGCTCC | siRNA |
| 383281-2MM | Il4R-α | TTTATTGACATAtAGCTgC | siRNA |
| 383281-4MM | Il4R-α | TTaATTcACATAtAGCTgC | siRNA |
| 383281-SCR | Il4R-α | agatcTctacgtctataat | siRNA |
| 459728 | Il4R-α | TAAAGACTTTATTGACATAA | siRNA |
| 459730 | Il4R-α | GACAAGATAAAGACTTTATT | siRNA |
| 459732 | Il4R-α | TATTGACATAAAGCTCCATG | siRNA |
| 451522 | Il4R-α | TTTATTGACATAAAGCTCC | siRNA |
| 455185 | Il4R-α | TTTATTGACATAAAGCTCC | siRNA |
| 29589 | PTEN | TTAAATTTGGCGGTGTCA | siRNA |
| 29592 | PTEN | TGTCTCTGGTCCTTACTT | siRNA |
| 29593 | PTEN | ACATAGCGCCTCTGACTG | siRNA |
| 29600 | PTEN | TGCTAGCCTCTGGATTTG | siRNA |
| 29604 | PTEN | GTGTCAAAACCCTGTGGA | siRNA |
| 371864 | Eg5 | CAACAAGGATGAAGTCTAT | siRNA |
| 549896 | WAF1 | TTTATTGAGCACCAGCTTTG | siRNA |
| 549894 | GATA3 | TTTAATAAATACCATCAAAA | siRNA |
| 549900 | STAT2 | TTTATTATGACGTACGAAGG | siRNA |
| 308299 | Exosc9 | TGCCAAACCTTTTCACCAGC | RNaseH |
| 454373 | SKIV2l | GCCAGCCTCATCCACATAGC | RNaseH |
| 395392 | Ago1 | CTGGACTATTTGCATAGCAA | RNaseH |
| 399560 | Ago3 | GTTTGCCCATAGTGCCATAC | RNaseH |
| 395927 | Ago4 | GGCTAACAGTCGAATTGGTT | RNaseH |
| 368473 | Dcp1a | CACCATGTTGCTCAGGGAGG | RNaseH |
| 365128 | DCP2 | CTGCATGTAGAAATCCAAGT | RNaseH |
| 443335 | GW182 | TCCTCAGTAGATTTCCATCC | RNaseH |
| 339800 | DDX6 | CTCGGGTAAACAGATCAGTG | RNaseH |
All siRNA are full RNA in both sense and antisense strand except underlined bases which indicate 2′-O-methoxyethyl substitutions (2′MOE). All RNaseH-dependent ASOs are full phosphorothioate with 2′-O-methoxyethyl substitutions at the positions indicated by underline. Nucleotide mismatches are represented by lower case.