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. 2012 Mar 19;40(13):5910–5925. doi: 10.1093/nar/gks244

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — HBZ binds the HAT domain of p300/CBP. (A) Schematic representation of CBP and the CBP fragments used in GST pull-down assays. (B) C-terminal truncation of the HAT domain disrupts HBZ-binding. HBZ was incubated with 10 pmol of GST, GST-HAT or GST-N-HAT. Proteins retained on glutathione beads were analyzed by SDS–PAGE and autoradiography. (C) HBZ interacts with a region of p300/CBP encompassing the C-terminal portion of the HAT domain and the ZZ domain, and separately with the TAZ-2 domain. HBZ was incubated with 10 pmol of the indicated GST fusion proteins. Proteins retained on the glutathione beads were analyzed by SDS–PAGE and autoradiography.