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. 2012 Mar 19;40(13):5910–5925. doi: 10.1093/nar/gks244

Figure 8.

Figure 8.

HBZ inhibits p65 and p53 acetylation by p300/CBP. (A) HBZ inhibits p65 acetylation by p300 in vitro. HAT assays were performed using recombinant p65 (80 nM), p300 (4 nM) and GST-HBZ (0.3 µM) or HBZ-bZIP (0.3 µM) as indicated. (B) HBZ inhibits p53 acetylation by p300 in vitro. HAT assays were performed using recombinant p53 (50 nM), p300 (4 nM) and GST-HBZ (0.5 µM) or HBZ-bZIP (0.5 µM) as indicated. In (A) and (B), the lower panel shows the membrane from the top panel reprobed for total p65 and p53, respectively. Reactions were analyzed by western blot using the antibodies indicated. (C) HBZ inhibits p65 acetylation in vivo. Nuclear extracts (40 µg) were prepared from HeLa cell lines expressing HBZ or containing the empty pcDNA vector. Cells used in these experiments were stimulated with 50 µM etoposide (ETO) for 5 h. (D) HBZ inhibits p53 acetylation in vivo. Experiments were performed as described in (C). In (C) and (D) the middle panel shows the membrane from the upper panel reprobed for total p65 and p53, respectively. Extracts were analyzed by western blot using the antibodies indicated.