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. 2012 Mar 20;40(13):6082–6096. doi: 10.1093/nar/gks255

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — The influence of HP-CE release by different cations. (A) FRET changes over the course of cation addition. The reaction was initiated with e/cRAG in Mg²⁺ and incubated for 2 h, after which Mg²⁺ (black), Ca²⁺ (magenta) or Mn²⁺ (blue) were added. A mock treatment is shown in cyan. (B). Denaturing gel electrophroresis analysis of HP-CE production after the step-wise addition of different cations. The different bands (from top to bottom) represent the intact RSS, hairpin and nicked DNA. All reactions were initiated by e/cRAG in Mg²⁺ and incubated for 2 h, after which the different cations described in the figure were added to the reaction. The times represent the incubation time after cation addition.