. 2012 Mar 20;40(13):6082–6096. doi: 10.1093/nar/gks255

Table 2.

Kinetics of the production and release of HP-CEs

Cleavage reaction	RAG	Cation	τ_HP-P^a (min)	τ_HP-R^b (min)
Single	c/cRAG	Mn²⁺	60 ± 5	120 ± 6
	e/cRAG	Mn²⁺	10 ± 2	τ₁ 12 ± 3; τ₂ = 370 ± 30^c
	e/cRAG	Mg²⁺	60 ± 4	400 ± 14
Coupled	c/cRAG	Mn²⁺	ND^d	186 ± 10
	c/cRAG	Mg²⁺	ND	>600
	e/cRAG	Mn²⁺	ND	τ₁ 12 ± 2; τ₂ = 380 ± 25^c
	e/cRAG	Mg²⁺	ND	>600
	c/cRAG^e	Mg²⁺	60 ± 4	231 ± 4.7
	c/flRAG^e	Mg²⁺	62 ± 3	198 ± 8.1
	c/flRAG + peptide^e	Mg²⁺	52 ± 4	255 ± 4.8

^aLifetime of HP-CE production, a measure of the rate of HP-CE production. The number given was calculated from the kinetic analysis of HP-CE production, as exemplified in Figures 2A and 7.

^bLifetime obtained from FRET or anisotropy, a measure of the rate of HP-CE release, as exemplified in Figures 2A and 7.

^cBi-phasic kinetics, in which τ₁ is relevant to HP-CE release whereas τ₂ may be caused by the generation of additional band as it is not correlated with τ_HP-P, i.e. HP-CE production rate (see Figure 2A).

^dNot determined.

^eThe cleavage reaction was conducted in the presence of 5% DMSO, which was found to cause reduction in τ_HP-P, as compared to the other listed reactions that were conducted in the absence of DMSO.