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. 2012 Mar 28;40(13):6049–6059. doi: 10.1093/nar/gks256

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — SUMO modification of PCNA reduces the accumulation of γH2AX foci. (A) HeLa cells stably expressing FLAG, FLAG-PCNA or FLAG-PCNA-SUMO1 were fixed 3 h after mock or (B) MMS treatments and stained with anti-FLAG (green), anti-γH2AX (red) and DAPI (blue). (C) Percentage of cell populations that showed more than two foci for γH2AX at 0.5, 1, 2, 3 and 5 h after MMS treatment was calculated from three independent experiments; error bars show standard deviations. (D) Effect of the expression of K164R PCNA in RAD18^−/− HCT116 cells was revealed by γH2AX staining 3 h after MMS treatment as described above.