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. 2012 Mar 28;40(13):6049–6059. doi: 10.1093/nar/gks256

Figure 6.

Figure 6.

SUMO modification of PCNA limits MMS-induced DNA DSB formation and effects DNA damage sensitivity. (A) Representative images of HeLa cells stably expressing FLAG, FLAG-PCNA or FLAG-PCNA-SUMO1 either untreated or 2 and 5 h after MMS treatment subjected to comet assay under neutral conditions. (B) Percentage of comet tail DNA, a measure of DNA DSBs, at 0.5, 1, 2, 3 and 5 h after MMS treatment were calculated from three independent experiments using three independent stable cell lines; error bars show standard deviations. Significant difference was detected between the levels of double stand breaks of FLAG FLAG-PCNA-SUMO1, FLAG-PCNA and FLAG-PCNA-SUMO1 expressing cell lines *P < 0.001). HeLa cells stably expressing FLAG, FLAG-PCNA and FLAG-PCNA-SUMO1 were assayed for cell survival after treatment with 0.005 and 0.01 µM MMS (C) or 10 and 150 µM Cisplatin (D) as compared to the untreated cells. Error bars show standard deviation from the results of three independent experiments.