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. 2012 Apr 5;40(13):5988–6000. doi: 10.1093/nar/gks286

Figure 6.

Figure 6.

Recruitment of the P-TEFb complex to the MYB regulatory region is dependent on ligand-bound ERα. (A,B) MCF-7 cells were cultured in estrogen-free medium for 48 h, following which 10 nM estrogen or vehicle was added for a further 16 h. Extracts prepared from cells cultured in both conditions were used to perform ChIP assays using (A) anti-ERα antibody (1.0 µg), (B) anti-CyclinT1 (0.5 µg) and anti-CDK9 (0.5 µg) antibodies and corresponding control antibody (IgG) or no antibody (mock). The amount of binding was estimated by qPCR and expressed as percent input. (C,D) MCF-7 cells were incubated with 10 µM ICI 182780 for 16 h (ICI). Half of these cells were harvested and the remaining cells were incubated in fresh drug-free growth medium for 24 h (Recovery). An extract prepared from MCF-7 cells grown in regular growth medium (RGM) was used as control. Extracts of harvested cells were used for ChIP assays using (C) anti-ERα (1.0 µg), (D) anti-CyclinT1 (0.5 µg) or anti-CDK9 (0.5 µg) antibodies as indicated.