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. 2012 Apr 5;40(13):5988–6000. doi: 10.1093/nar/gks286

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Estrogen stimulation selectively induces P-TEFb complex-driven Ser-2 phosphorylation of RNA Pol II in the MYB attenuation site. ChIP assays were performed with MCF-7 extract from cells grown either in the absence or presence of estrogen using anti-RNA Pol II antibody or anti-RNA Pol II CTD phospho Ser-2 antibody. The amount of total RNA Pol II and Ser-2 phospho-Pol II bound was detected by qPCR using MYB intron-1-specific primers (A) and pS2 promoter-specific primers (B), and expressed with respect to the input. These data are also plotted (C) to show the proportion of Ser-2 phosphorylated RNA Pol II compared with the total RNA Pol II present at the pS2 promoter and MYB intron-I pausing site in the presence or absence of estrogen.