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. Author manuscript; available in PMC: 2012 Jul 21.
Published in final edited form as: Dev Dyn. 2009 Sep;238(9):2309–2317. doi: 10.1002/dvdy.21974

Figure 1.

Figure 1

Generation of epitope-tagged alleles. (A) Structures of wild-type Math5 and Math5HA alleles. Sequences encoding three copies of HA tag were fused in frame with the coding region of Math5 in Math5HA. (B) Structures of wild-type Pou4f2 and Pou4f2HA alleles. In diagrams of A and B, blue boxes are coding regions, green ovals are HA tags, brown boxes are Neo cassettes (eventually deleted by crossing with the CMV-Cre line as indicated by the red crosses), and red triangles are loxP sites. B is a restriction site for BamH I. Positions of external probes and the sizes of DNA fragments that are recognized in different alleles are indicated. (C) Southern blot hybridization to identify ES cell clones harboring the targeted alleles. Wild-type (+) and targeted bands (HA) for each allele are indicated. Positive clones have two bands.