Table 2.
Apoptotic (ApI), oncotic (OI) indexes of HEp-2 cells infected with Acb complex strains at 24 and 48 h with and without the pan-caspase inhibitor, extracellular and cell-contact cytotoxicity, adhesion (AdI), and invasion (InI) indexes
| Strain no. | ApI (%) | OnI (%) | Extracellular cytotoxic activity | Cell-contact cytotoxicity (%) | AdI [×105] | InI (%) |
|---|---|---|---|---|---|---|
| A. calcoaceticus | ||||||
| MPU M12 | 65.1a/61.2 | 11.6c/17.8 | 32e | 71.3f | 25.4 g | 0.07 h |
| 67.1b/58.7 | 21.3d/16.4 | |||||
| MPU M5 | 61.3/57.1 | 8.7/6.8 | 32 | 72.4 | 24.3 | 3.1 |
| 78.6/73.1 | 15.4/11.6 | |||||
| MPU M13 | 56.3/37.1 | 7.9/6.8 | 8 | 65.4 | 27.6 | 0.1 |
| 67.6/42.7 | 18.7/14.1 | |||||
| MPU M4 | 56.1/61.4 | 11.6/12.8 | 1 | 69.8 | 21.6 | 1.2 |
| 60.3/57.1 | 21.4/18.7 | |||||
| MPU M9 | 50.7/29.2 | 4.8/3.6 | 0 | 54.6 | 21.9 | 11.5 |
| 59.9/31.4 | 9.4/7.4 | |||||
| MPU M6 | 43.4/47.6 | 50.3/45.6 | 0 | 59.7 | 18.8 | 57.8 |
| 36.1/40.7 | 45.1/41.3 | |||||
| MPU M19 | 35.4/38.1 | 51.4/54.3 | 0 | 39.6 | 20.7 | 51.3 |
| 44.8/39.1 | 53.7/49.6 | |||||
| MPU M8 | 18.6/23.2 | 49.2/45.7 | 0 | 51.4 | 19.6 | 61.2 |
| 28.3/31.2 | 56.1/55.8 | |||||
| MPU M17 | 16.1/18.3 | 8,5/4.2 | 1 | 31.2 | 17.4 | 0.8 |
| 29.4/31.6 | 12.6/9.8 | |||||
| MPU M7 | 14.6/7.8 | 51.6/54.7 | 0 | 68.5 | 19.7 | 49.3 |
| 21.4/12.7 | 67.9/64.2 | |||||
| A. baumannii | ||||||
| MPU M25 | 58.1/53.2 | 11.4/7.2 | 0 | 61.3 | 23.6 | 0.08 |
| 65.4/61.7 | 18.9/9.7 | |||||
| MPU M20 | 57.4/37.8 | 7.8/5.4 | 8 | 64.2 | 21.4 | 0.09 |
| 65.7/39.1 | 18.5/14.1 | |||||
| MPU M22 | 57.3/31.8 | 11.4/8.7 | 1 | 58.6 | 23.7 | 1.8 |
| 68.4/38.7 | 21.7/15.3 | |||||
| MPU M21 | 56.1/29.7 | 21.3/11.4 | 4 | 57.1 | 28.5 | 2.7 |
| 66.8/49.3 | 28.3/21.7 | |||||
| MPU M24 | 38.6/13.2 | 5.9/5.1 | 32 | 48.3 | 23.8 | 0.6 |
| 54.8/21.7 | 12.9/7.8 | |||||
| MPU M23 | 27.3/9.8 | 51.1/61.2 | 1 | 61.4 | 15.7 | 52.8 |
| 38.7/19.6 | 59.7/68.3 | |||||
| MPU M16 | 32.1/29.3 | 48.1/51.6 | 0 | 42.1 | 19.1 | 58.1 |
| 34.7/26.8 | 57.5/61.2 | |||||
aThe percentage of apoptotic cells at 24 h without pan-caspase inhibitor/with the inhibitor
bThe percentage of apoptotic cells at 48 h after infection without the pan-caspase inhibitor/with the inhibitor
cThe percentage of oncotic cells at 24 h without the pan-caspase inhibitor/with the inhibitor
dThe percentage of oncotic cells at 48 h after infection without the pan-caspase inhibitor/with the inhibitor
eThe reciprocal of the highest dilution of supernatant yielding rounding, detachment and destruction of 50 % of a HEp-2 monolayer at 24 h
fThe percentage of cytotoxicity was determined by MTT at 5 h
gThe mean total number of CFU associated bacteria per well
hThe percentage of intracellular bacteria after gentamicin treatment in comparison to initial inoculum. All values correspond to the means from two experiments in triplicate