Fig. 2.

Tumor-specific recognition of MLTCs from patient #2710 cultured in vitro in the presence of different cytokines or of their combinations. The specific tumor recognition of lymphocytes isolated from PBMCs of the cutaneous melanoma patient 2710 and cultured for 2 weeks in the presence of different cytokines was analyzed for IFN-γ release (ELISPOT assay). IFN-γ secretion by T cells was assessed following the incubation with the autologous melanoma line (#2710 mel) pre-treated or not with anti-HLA class I (W6/32) or anti-HLA class II (L243) mAbs. The recognition of the allogeneic HLA-matched (HLA-A*0201⁺ 501 mel) or HLA-mismatched (15392 mel) melanoma lines and of the NK target cell line K562 was also determined. PHA/Con-A was used as a positive control for IFN-γ release. Statistical analysis of differences between means of IFN-γ released by T cells was done by two-tailed t test (P ≤ 0.01)