Skip to main content
. Author manuscript; available in PMC: 2012 Jul 22.
Published in final edited form as: ACS Chem Biol. 2011 Apr 5;6(6):658–666. doi: 10.1021/cb200017n

Figure 3. Selectivity of newly engineered peptide inhibitors towards JNK2.

Figure 3

a) JIP10-Δ-TATi is a potent and selective inhibitor towards JNK2, with an IC50 ~ 92 nM as determined by fitting the fractional activity at every inhibitor concentration to Morrison’s equation for a tight-binding inhibitor (35). b) JIP10-Δ-TATi exhibits 10-fold greater selectivity for JNK2 compared to JNK1 and JNK3, while maintaining limited potency towards p38MAPKα (150-fold less than JNK2) and ERK2. c) JIP10-Δ-R9 exhibits an IC50 of ~89 nM towards JNK2 (fitting to Morrison’s equation (35) for tight-binding inhibitor). d) JIP10-Δ-R9 exhibits a 10-fold higher selectivity for JNK2 over JNK1 and JNK3 with limited potency towards p38MAPKα and ERK2.